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Title: Anti‑EpCAM antibodies for detection of metastatic carcinoma in effusions and peritoneal wash
Authors: Carneiro, Fabiana Pirani
Muniz‑Junqueira, Maria Imaculada
Carneiro, Marcos de Vasconcelos
Oliveira, Ísis de Araújo
Soares, Aluízio Carlos
Haar, Nathália de Vargas
Takano, Gustavo Henrique Soares
Vianna, Leonora Maciel de Sousa
Caldas, Guilherme de Carvalho
Vieira, Danillo Leal Marinho
Frutuoso, Lígia Lins
Brito, Larissa Matos Rodrigues
Siqueira, Rafael Vieira Martins de
Parente, Amanda Moreira
Castro, Tercia Maria Mendes Lousa de
Peres, Isabela
Mendes, Lianna Martha Soares
Borges, Tatiana Karla dos Santos
Ferreira, Vânia Moraes
Motoyama, Andrea Barretto
Assunto:: Câncer
Issue Date: 2019
Publisher: Spandidos Publications
Citation: CARNEIRO, Fabiana Pirani et al. Anti‑EpCAM antibodies for detection of metastatic carcinoma in effusions and peritoneal wash. Oncology y Letters, v. 18, p. 2019-2024, 2019. DOI: 10.3892/ol.2019.10468. Disponível em: Acesso em: 02 dez. 2019.
Abstract: Epithelial cell adhesion molecule (EpCAM) has been used as diagnostic/prognostic marker and therapeutic target. The aim of the present study was to compare immunoreactivity of antibodies against distinct epitopes in the ectodomain of EpCAM for detection of carcinoma from different primary sites and of different histological types in effusions and peritoneal wash. Two antibodies against epitopes in the EGF‑like domain I (clones Moc‑31 and Ber‑EP4) and one antibody against the epitope in the cysteine‑poor region (158210) of EpCAM were used (all commercially available). Independently of the clone used, EpCAM overexpression was observed in almost all samples when all the adenocarcinoma samples were analyzed together. By using Moc‑31, EpCAM overexpression was observed in all samples of adenocarcinoma. Absence of EpCAM overexpression was observed in a few adenocarcinoma samples at some sites of tumor origin, including ovary, breast and stomach, when Ber‑EP4 and 158210 were used. Regarding carcinomas aside from adenocarcinomas, histological types, such as squamous cell, urothelial and small cell carcinoma showed different degrees of EpCAM expression according to the antibody used. In squamous cell carcinoma, overexpression was observed only with the clone 158210. It was concluded that, overall, most samples of metastatic carcinoma from effusions showed overexpression of EpCAM. However, there are significant variations in its detection according to the primary site, histological type of the carcinoma and depending on the antibody used. Thus, the use of more than one type of anti‑EpCAM antibody would increase the chance of its detection in metastatic carcinoma effusion.
DOI: 10.3892/ol.2019.10468
Appears in Collections:FMD - Artigos publicados em periódicos e preprints

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