| DC Field | Value | Language |
|---|
| dc.contributor.author | Thiebaut, Flávia | - |
| dc.contributor.author | Rojas, Cristian A. | - |
| dc.contributor.author | Grativol, Clícia | - |
| dc.contributor.author | Calixto, Edmundo P. da R. | - |
| dc.contributor.author | Motta, Mariana R. | - |
| dc.contributor.author | Ballesteros, Helkin G. F. | - |
| dc.contributor.author | Peixoto, Barbara | - |
| dc.contributor.author | Lima, Berenice N. S. de | - |
| dc.contributor.author | Vieira, Lucas M. | - |
| dc.contributor.author | Walter, Maria Emília Machado Telles | - |
| dc.contributor.author | Armas, Elvismary M. de | - |
| dc.contributor.author | Entenza, Júlio O. P. | - |
| dc.contributor.author | Lifschitz, Sergio | - |
| dc.contributor.author | Farinelli, Laurent | - |
| dc.contributor.author | Hemerly, Adriana Silva | - |
| dc.contributor.author | Ferreira, Paulo Cavalcanti Gomes | - |
| dc.date.accessioned | 2018-06-26T14:57:05Z | - |
| dc.date.available | 2018-06-26T14:57:05Z | - |
| dc.date.issued | 2017-12-20 | - |
| dc.identifier.citation | THIEBAUT, Flávia et al. Roles of non-coding RNA in sugarcane-microbe interaction. Non-coding RNA, v. 3, n. 4, 2013. Disponível em: <http://www.mdpi.com/2311-553X/3/4/25>. Acesso em: 26 jun. 2018. doi: https://doi.org/10.3390/ncrna3040025. | pt_BR |
| dc.identifier.uri | http://repositorio.unb.br/handle/10482/32109 | - |
| dc.language.iso | Inglês | pt_BR |
| dc.publisher | MDPI | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Roles of non-coding RNA in sugarcane-microbe interaction | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | Ácido ribonucléico | pt_BR |
| dc.subject.keyword | Bactérias | pt_BR |
| dc.subject.keyword | Cana-de-açúcar | pt_BR |
| dc.subject.keyword | Biologia computacional | pt_BR |
| dc.rights.license | © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | pt_BR |
| dc.identifier.doi | https://doi.org/10.3390/ncrna3040025 | pt_BR |
| dc.description.abstract1 | Studies have highlighted the importance of non-coding RNA regulation in plant-microbe
interaction. However, the roles of sugarcane microRNAs (miRNAs) in the regulation of disease
responses have not been investigated. Firstly, we screened the sRNA transcriptome of sugarcane
infected with Acidovorax avenae. Conserved and novel miRNAs were identified. Additionally,
small interfering RNAs (siRNAs) were aligned to differentially expressed sequences from the
sugarcane transcriptome. Interestingly, many siRNAs aligned to a transcript encoding a coppertransporter
gene whose expression was induced in the presence of A. avenae, while the siRNAs were
repressed in the presence of A. avenae. Moreover, a long intergenic non-coding RNA was identified
as a potential target or decoy of miR408. To extend the bioinformatics analysis, we carried out
independent inoculations and the expression patterns of six miRNAs were validated by quantitative
reverse transcription-PCR (qRT-PCR). Among these miRNAs, miR408—a copper- microRNA—was
downregulated. The cleavage of a putative miR408 target, a laccase, was confirmed by a modified
50RACE (rapid amplification of cDNA ends) assay. MiR408 was also downregulated in samples
infected with other pathogens, but it was upregulated in the presence of a beneficial diazotrophic
bacteria. Our results suggest that regulation by miR408 is important in sugarcane sensing whether
microorganisms are either pathogenic or beneficial, triggering specific miRNA-mediated regulatory
mechanisms accordingly. | pt_BR |
| Appears in Collections: | Artigos publicados em periódicos e afins
|
