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Title: Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction : systematic literature review and meta-analysis
Authors: Gomes, Ciro Martins
Mazin, Suleimy Cristina
Santos, Elisa Raphael dos
Cesetti, Mariana Vicente
Bächtold, Guilherme Albergaria Brízida
Cordeiro, João Henrique de Freitas
Theodoro, Fabrício Claudino Estrela Terra
Damasco, Fabiana dos Santos
Carranza, Sebastián Andrés Vernal
Santos, Adriana de Oliveira
Roselino, Ana Maria
Sampaio, Raimunda Nonata Ribeiro
Assunto:: Leishmaniose
Issue Date: Apr-2015
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: GOMES, Ciro Martins et al. Accuracy of mucocutaneous leishmaniasis diagnosis using polymerase chain reaction: systematic literature review and meta-analysis. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 110, n. 2, p. 157-165, abr. 2015. Disponível em: <>. Acesso em: 9 maio 2018. Epub Mar 31, 2015. doi:
Abstract: The diagnosis of mucocutaneous leishmaniasis (MCL) is hampered by the absence of a gold standard. An accurate diagnosis is essential because of the high toxicity of the medications for the disease. This study aimed to assess the ability of polymerase chain reaction (PCR) to identify MCL and to compare these results with clinical research recently published by the authors. A systematic literature review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses: the PRISMA Statement was performed using comprehensive search criteria and communication with the authors. A meta-analysis considering the estimates of the univariate and bivariate models was performed. Specificity near 100% was common among the papers. The primary reason for accuracy differences was sensitivity. The meta-analysis, which was only possible for PCR samples of lesion fragments, revealed a sensitivity of 71% [95% confidence interval (CI) = 0.59; 0.81] and a specificity of 93% (95% CI = 0.83; 0.98) in the bivariate model. The search for measures that could increase the sensitivity of PCR should be encouraged. The quality of the collected material and the optimisation of the amplification of genetic material should be prioritised.
Licença:: Memórias do Instituto Oswaldo Cruz - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY NC 4.0). Fonte: Acesso em: 9 maio 2018.
Appears in Collections:FMD - Artigos publicados em periódicos e preprints

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