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Title: Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil
Authors: Amorim, Regina MS
Oliveira, Claudiner P
Wyant, Patrícia S
Cerqueira, Daniela M
Câmara, Geni NL
Flores, Luciano S
Martins, Regina MB
Martins, Cláudia RF
Assunto:: hepatitis C virus
Federal District, Central Brazil
Issue Date: 2004
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Mem. Inst. Oswaldo Cruz,v.99,n.8,p.895-897,2004
Abstract: The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.
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