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Title: Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission
Authors: Romero, Gustavo Adolfo Sierra
Sampaio, Raimunda Nonata Ribeiro
Macêdo, Vanize de Oliveira
Marsden, Philip Davis
Assunto:: Leishmania (Viannia) braziliensis
culture sensitivity
fungal contamination
vacuum aspiratory technique
Issue Date: 1999
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Mem. Inst. Oswaldo Cruz,v.94,n.4,p.505-508,1999
Abstract: Sixty eight patients with localized cutaneous leishmaniasis from an area with Leishmania (Viannia) braziliensis transmission had cultures performed with a modified Marzochi´s vacuum aspiratory puncture technique to establish sensitivity and contamination rate with this new method. Overall sensitivity of three aspirates was 47.1%; (CI95% 39.4; 59.4) significantly greater than the sensitivity of a single one aspirate. Fungal contamination was observed in 6/204 (2.9%) inoculated culture tubes. We recommend that this useful technique should be adopted as routine for primary isolation of L. (V.) braziliensis from localized cutaneous ulcers.
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