Título:	Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
Autor(es):	Diniz, Sandoval Felicíssimo Amorim, Fernanda Penna Lima Guedes de Cavalcante Neto, Florêncio Figueiredo Bocca, Anamélia Lorenzetti Batista, Aline Carvalho Simm, G. E. P. M. Silva, Tarcília Aparecida da
Afiliação do autor:	Universidade de Brasília, Faculdade de Medicina Universidade de Brasília, Faculdade de Medicina Universidade de Brasília, Faculdade de Medicina Universidade de Brasília, Instituto de Biologia, Departamento de Biologia Celular Universidade Federal de Goiás, Faculdade de Odontologia, Departamento de Estomatologia (Patologia Oral) Universidade Federal de Minas Gerais, Faculdade de Odontologia, Departamento de Clínica, Patologia e Cirurgia Odontológicas Universidade Federal de Minas Gerais, Faculdade de Odontologia, Departamento de Clínica, Patologia e Cirurgia Odontológicas
Assunto:	Tíbia - fraturas Tíbia - ferimentos e lesões Diabetes
Data de publicação:	2008
Editora:	Associação Brasileira de Divulgação Científica
Referência:	DINIZ, S.F. et al. Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture. Brazilian Journal of Medical and Biological Research, v. 41, n. 5, p. 373-379, 2008. DOI: http://dx.doi.org/10.1590/S0100-879X2008005000014. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500005&lng=en&tlng=en. Acesso em: 7 jul. 2017. doi: http://dx.doi.org/10.1590/S0100-879X2008005000014 .
Abstract:	A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to controls. Radiographic analysis revealed a displaced fracture with poor bone fragment alignment and delayed consolidation at these times in the diabetic group. The levels of alkaline phosphatase were significantly higher in diabetic rats at 25 days (P = 0.009). These results suggest that the initial excessive formation of fibrous connective tissue associated with delay in chondrogenesis and osteogenesis may not provide suitable stability of the fractured site, contributing to the inappropriate alignment of fragments and an increase in the volume of callus in later stages of repair. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation.
Unidade Acadêmica:	Faculdade de Medicina (FM) Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
Licença:	Journal of Medical and Biological Research - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons.
DOI:	http://dx.doi.org/10.1590/S0100-879X2008005000014
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