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Title: ScFv from antibody that mimics gp43 modulates the cellular and humoral immune responses during Experimental Paracoccidioidomycosis
Authors: Jannuzzi, Grasielle Pereira
Tavares, Aldo Henrique Fonseca Pacheco
Kaihami, Gilberto Hideo
Almeida, José Roberto Fogaça de
Almeida, Sandro Rogério de
Ferreira, Karen Spadari
Assunto:: Micoses
Paracoccidioidomicose
Células
Issue Date: 19-Jun-2015
Publisher: Plos One
Citation: JANNUZZI, Grasielle Pereira et al. ScFv from antibody that mimics gp43 modulates the cellular and humoral immune responses during Experimental Paracoccidioidomycosis. Plos One, v. 10, n. 6, Article e0129401, 19 jun. 2015. Disponível em: <http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129401>. Acesso em: 14 jun. 2017. doi: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0129401.
Abstract: Paracoccidioidomycosis (PCM), caused by Paracoccidioides species is a prevalent systemic and progressive mycosis that occurs in Latin America. It is caused by Paracoccidioides species. Immunization with dendritic cells transfected with a plasmid encoding the scFv (pMAC/PS-scFv) that mimics the main antigen of P. brasiliensis (gp43) confers protection in experimental PCM. DCs link innate and adaptive immunity by recognizing invading pathogens and selecting the type of effector T cell to mediate the immune response. Here, we showed that DC-pMAC/PS-scFv induces the activation of CD4+ and CD8+ T cells. Moreover, our results demonstrated that BALB/c mice infected with P. brasiliensis and treated with DC-pMAC/PS-scFv showed the induction of specific IgG production against gp43 and IFN-γ, IL-12 and IL-4 cytokines. Analysis of regional lymph nodes revealed increases in the expression of clec7a, myd88, tlr2, gata3 and tbx21, which are involved in the immune response. Taken together, our results indicate that the scFv modulates the humoral and cellular immune responses and presents epitopes to CD4+ and CD8+ T cells.
Licença:: Copyright: © 2015 Jannuzzi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: https://dx.doi.org/10.1371/journal.pone.0129401
Appears in Collections:CEL - Artigos publicados em periódicos

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