Title:	Genômica estrutural e funcional de fungos do gênero Trichoderma Genômica estrutural e funcional de fungos do gênero Trichoderma
Authors:	Steindorff, Andrei Stecca
Orientador(es)::	Noronha, Eliane Ferreira
Coorientador(es)::	Pappas Júnior, Georgios Joannis Grigoriev, Igor
Assunto::	Trichoderma harzianum Fungos - genômica comparativa Controle biológico Fungos fitopatogênicos - controle
Issue Date:	28-Apr-2016
Data de defesa::	16-Mar-2016
Citation:	STEINDORFF, Andrei Stecca. Genômica estrutural e funcional de fungos do gênero Trichoderma. 2016. 96 f., il. Tese (Doutorado em Biologia Molecular)—Universidade de Brasília, Brasília, 2016.
Abstract:	Biological control is a complex process, which requires many mechanisms and a high diversity of biochemical pathways. Trichoderma harzianum species complex are well known for their biocontrol activity against many plant pathogens. To gain new insights into the biocontrol mechanism employed by T. harzianum, we sequenced genome of the isolate TR274 with its transcriptome during direct interaction with the fungal pathogen Sclerotinia sclerotiorum and its cell wall, using Illumina sequencing. Whole genome assembly was performed using AllPaths-LG, with a maximum coverage of 100x. The assembly resulted in 2282 contigs, with an estimated genome size of 40.8 Mb and GC content of 47.7%, similar to other Trichoderma genomes. Using the JGI Annotation Pipeline we predicted 13,932 genes, with high transcriptome support. Core Eukariotic Genes Dataset (CEGMA) tests suggested 100% genome completeness and 97.9% of RNA-SEQ reads mapped to the genome. The phylogenetic comparison using orthologous proteins with all Trichoderma genomes sequenced at JGI, corroborates the Trichoderma section division described previously (T. asperellum and T. atroviride), Longibrachiatum (T. reesei, T. citrinoviride and T. longibrachiatum) and Pachibasium (T. harzianum and T. virens). A Venn diagram was built with orthologs proteins, with 8242 composing the core protein group and species specific varying from 262 proteins (T. reesei) to 1803 (T. longibrachiatum). The comparison between two Trichoderma harzianum CBS 226.95 and TR274 isolates, suggests a high genome similarity. Analyses of the secondary metabolites, CAZymes, transporters, proteases and transcription factors were performed. The Pachybasium section expanded virtually all categories analyzed compared with the other sections. CAFE analysis showed positive correlation between these families and genome size. The chitinase subgroup C1 was completely absent in Longibrachiatum section members. These results suggest that these proteins families play an important role on their respective phenotypes. Transcriptome analysis suggests that the interaction between T. harzianum and S. sclerotiorum is complex, involving production of secondary metabolites and transporters before and during the contact, with a modulation of CAZymes after contact. A total of 86.8% of differentially expressed genes during growth on Sclerotinia sclerotiorum cell wall were found during direct interaction. Approximately the 25% of whole T. harzianum genome was seen to be modulated during the interaction with S. sclerotiorum.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
Description:	Tese (doutorado)—Universidade de Brasília, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Biologia Molecular, 2016.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Biologia Molecular
Licença::	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições:Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
DOI:	http://dx.doi.org/10.26512/2016.03.T.20049
Appears in Collections:	Teses, dissertações e produtos pós-doutorado

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