Título:	Hepatoprotective effects of four Brazilian savanna species on acetaminophen-induced hepatotoxicity in HepG2 cells
Autor(es):	Ribeiro, Gislane dos Santos Martins, Diegue Henrique Nascimento Gomes, João Victor Dutra Davies, Noel William Fagg, Christopher William Simeoni, Luiz Alberto Homem de Mello, Mauricio Batista, Pérola de Oliveira Magalhães Dias Silveira, Dâmaris Fonseca-Bazzo, Yris Maria
ORCID:	https://orcid.org/0000-0001-6098-3852 https://orcid.org/0000-0002-4541-9177 https://orcid.org/0000-0002-4541-9177 https://orcid.org/0000-0001-8011-6940 https://orcid.org/0000-0002-1230-3207
Afiliação do autor:	University of Brasília, Health Sciences School, Pharmacy Department University of Brasília, Health Sciences School, Pharmacy Department University of Brasília, Health Sciences School, Pharmacy Department University of Tasmania, Central Science Laboratory University of Brasília, Institute of Biological Science, Department of Botany University of Brasília, Health Sciences School, Pharmacy Department University of Brasília, Health Sciences School, Pharmacy Department University of Brasília, Health Sciences School, Pharmacy Department University of Brasília, Health Sciences School, Pharmacy Department University of Brasília, Health Sciences School, Pharmacy Department
Assunto:	Paracetamol Atividade hepatotoxicidade Fitoterapia Atividade hepatoprotetora
Data de publicação:	26-Set-2023
Editora:	MDPI
Referência:	RIBEIRO, Gislane dos Santos et al. Hepatoprotective effects of four Brazilian savanna species on acetaminophen-induced hepatotoxicity in HepG2 cells. Plants, [S. l.], v. 12, n. 19, 3393, 2023. DOI: https://doi.org/10.3390/plants12193393. Disponível em: https://www.mdpi.com/2223-7747/12/19/3393. Acesso em: 06 fev. 2024.
Abstract:	We investigated four Cerrado plant species, i.e., Cheiloclinium cognatum (Miers) A.C.Sm, Guazuma ulmifolia Lam., Hancornia speciosa Gomes, and Hymenaea stigonocarpa Mart. ex Hayne, against acetaminophen toxicity using an in vitro assay with HepG2 cells. The activity against acetaminophen toxicity was evaluated using different protocols, i.e., pre-treatment, co-treatment, and post-treatment of the cells with acetaminophen and the plant extracts. HepG2 cell viability after treatment with acetaminophen was 39.61 ± 5.59% of viable cells. In the pre-treatment protocol, the extracts could perform protection with viability ranging from 50.02 ± 15.24% to 78.75 ± 5.61%, approaching the positive control silymarin with 75.83 ± 5.52%. In the post-treatment protocol, all extracts and silymarin failed to reverse the acetaminophen damage. In the co-treatment protocol, the extracts showed protection ranging from 50.92 ± 11.14% to 68.50 ± 9.75%, and silymarin showed 77.87 ± 4.26%, demonstrating that the aqueous extracts of the species also do not increase the toxic effect of acetaminophen. This protection observed in cell viability was accompanied by a decrease in ROS. The extracts’ hepatoprotection can be related to antioxidant compounds, such as rutin and mangiferin, identified using HPLC-DAD and UPLC-MS/MS. The extracts were shown to protect HepG2 cells against future APAP toxicity and may be candidates for supplements that could be used to prevent liver damage. In the concomitant treatment using the extracts with APAP, it was demonstrated that the extracts do not present a synergistic toxicity effect, with no occurrence of potentiation of toxicity. The extracts showed considerable cytoprotective effects and important antioxidant characteristics.
Unidade Acadêmica:	Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR) Instituto de Ciências Biológicas (IB) Departamento de Botânica (IB BOT)
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências Farmacêuticas
Licença:	Copyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
DOI:	https://doi.org/10.3390/plants12193393
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