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dc.contributor.authorBarbosa, Eder Alves-
dc.contributor.authorPlácido, Alexandra-
dc.contributor.authorMoreira, Daniel Carneiro-
dc.contributor.authorAlbuquerque, Lucas Fraga Friaça-
dc.contributor.authorDematei, Anderson-
dc.contributor.authorCarvalho, Amandda Évelin Silva de-
dc.contributor.authorCabral, Wanessa F.-
dc.contributor.authorBáo, Sônia Nair-
dc.contributor.authorAraújo, Felipe Saldanha de-
dc.contributor.authorKuckelhaus, Selma Aparecida Souza-
dc.contributor.authorBorges, Tatiana K.-
dc.contributor.authorPortugal, Camila C.-
dc.contributor.authorSocodato, Renato-
dc.contributor.authorTeixeira, Cátia-
dc.contributor.authorLima, Filipe Camargo D. A.-
dc.contributor.authorBatagin-Neto, Augusto-
dc.contributor.authorSebben, Antônio-
dc.contributor.authorEaton, Peter-
dc.contributor.authorGomes, Paula-
dc.contributor.authorBrand, Guilherme D.-
dc.contributor.authorRelvas, João B.-
dc.contributor.authorKato, Massuo J.-
dc.contributor.authorLeite, José Roberto de Souza de Almeida-
dc.date.accessioned2022-02-07T13:38:40Z-
dc.date.available2022-02-07T13:38:40Z-
dc.date.issued2021-11-10-
dc.identifier.citationBARBOSA, Eder Alves et al. The peptide secreted at the water to land transition in a model amphibian has antioxidant effects. Proceedings B, v. 288, n. 1962, 10 nov. 2021. DOI: https://doi.org/10.1098/rspb.2021.1531. Disponível em: https://royalsocietypublishing.org/doi/10.1098/rspb.2021.1531.pt_BR
dc.identifier.urihttps://repositorio.unb.br/handle/10482/42820-
dc.language.isoInglêspt_BR
dc.publisherThe Royal Societypt_BR
dc.rightsAcesso Abertopt_BR
dc.titleThe peptide secreted at the water to land transition in a model amphibian has antioxidant effectspt_BR
dc.typeArtigopt_BR
dc.subject.keywordAnfíbiopt_BR
dc.subject.keywordTriptofilinaspt_BR
dc.subject.keywordPeptídeospt_BR
dc.subject.keywordEspectrometria de massapt_BR
dc.subject.keywordStress oxidativopt_BR
dc.identifier.doihttps://doi.org/10.1098/rspb.2021.1531pt_BR
dc.relation.publisherversionhttps://royalsocietypublishing.org/doi/10.1098/rspb.2021.1531pt_BR
dc.description.abstract1In addition to the morphophysiological changes experienced by amphibians during metamorphosis, they must also deal with a different set of environmental constraints when they shift from the water to the land. We found that Pithecopus azureus secretes a single peptide ([M + H]+ = 658.38 Da) at the developmental stage that precedes the onset of terrestrial behaviour. De novo peptide and cDNA sequencing revealed that the peptide, named PaT-2, is expressed in tandem and is a member of the tryptophyllins family. In silico studies allowed us to identify the position of reactive sites and infer possible antioxidant mechanisms of the compounds. Cell-based assays confirmed the predicted antioxidant activity in mammalian microglia and neuroblast cells. The potential neuroprotective effect of PaT-2 was further corroborated in FRET-based live cell imaging assays, where the peptide prevented lipopolysaccharide-induced ROS production and glutamate release in human microglia. In summary, PaT-2 is the first peptide expressed during the ontogeny of P. azureus, right before the metamorphosing froglet leaves the aquatic environment to occupy terrestrial habitats. The antioxidant activity of PaT-2, predicted by in silico analyses and confirmed by cell-based assays, might be relevant for the protection of the skin of P. azureus adults against increased O2 levels and UV exposure on land compared with aquatic environments.pt_BR
dc.description.unidadeFaculdade de Medicina (FMD)-
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