Quantitative ¹H NMR spectroscopy (qNMR) in the early process development of a new quorum sensing inhibitor

Cavalcante, Robson Alves Fernandes; Silva, Felipe L.; Favero, Fernanda; Resck, Inês Sabioni; Pereira, Alex Leite; Machado, Angelo Henrique de Lira

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DC Field	Value	Language
dc.contributor.author	Cavalcante, Robson Alves Fernandes	-
dc.contributor.author	Silva, Felipe L.	-
dc.contributor.author	Favero, Fernanda	-
dc.contributor.author	Resck, Inês Sabioni	-
dc.contributor.author	Pereira, Alex Leite	-
dc.contributor.author	Machado, Angelo Henrique de Lira	-
dc.date.accessioned	2021-07-28T02:42:37Z	-
dc.date.available	2021-07-28T02:42:37Z	-
dc.date.issued	2020	-
dc.identifier.citation	CAVALCANTE, Robson Alves Fernandes. Quantitative ¹H NMR spectroscopy (qNMR) in the early process development of a new quorum sensing inhibitor. Magnetic Resonance in Chemistry, v. 58, p. 31-40, 2020. DOI: https://doi.org/10.1002/mrc.4906.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/41512	-
dc.language.iso	Inglês	pt_BR
dc.publisher	Wiley	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.title	Quantitative ¹H NMR spectroscopy (qNMR) in the early process development of a new quorum sensing inhibitor	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Controle de processo	pt_BR
dc.subject.keyword	Desenvolvimento de processo	pt_BR
dc.subject.keyword	Ressonância magnética nuclear	pt_BR
dc.identifier.doi	https://doi.org/10.1002/mrc.4906	pt_BR
dc.relation.publisherversion	https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/mrc.4906	pt_BR
dc.description.abstract1	2-methyl-5,6,7,8-tetrahydro-2H-chromen-4(3H)-one (called 6-oxo) is presented as a new AI-1 quorum sensing inhibitor for Vibrio harveyi. The development of a chemical process to afford traceable materials for new biological assays demands the development of analytical methods to ensure their purity and quality. This work describes the use of quantitative 1H nuclear magnetic resonance (NMR) spectroscopy (qNMR) to assess the purity of a sample of 6-oxo (99.88%) and a sample of its major process impurity (E)-1-(2-hydroxycyclohex-2-en-1-yl)but-2-en-1-one (called HCB; 98.28%). To explore the scope of the use of qNMR to quantify the amount of low-content components in samples related to the chemical process for 6-oxo synthesis, this work also determined the amount of 6-oxo in two HCB samples: (a) the high-purity HCB sample described above and (b) a crude HCB sample collected during the chemical process. Despite the complexity of the crude sample, the amount of 6-oxo was readily assessed and could help to estimate the extent to which 6-oxo was already formed during the HCB synthesis. This information can help the understanding of how the process parameters can be modified to improve the performance of the whole process, by controlling the reaction mechanisms working at each step of this chemical process. In this context, our results reinforce qNMR as a complementary analytical tool for the quantification of the main component found in a sample, contributing to the standardization of reference materials and thus allowing the development of analytical methods for process control and traceability of the samples used for biological assays.	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0002-0537-2482	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0002-0346-7146	pt_BR
dc.identifier.orcid	https://orcid.org/0000-0003-4284-9929	pt_BR
dc.description.unidade	Instituto de Química (IQ)	pt_BR
Appears in Collections:	Artigos publicados em periódicos e afins