| Campo DC | Valor | Idioma |
|---|
| dc.contributor.author | Almeida, Simoneide Souza Titze de | - |
| dc.contributor.author | Horst, Camila Hillesheim | - |
| dc.contributor.author | Sánchez, Cristina Soto | - |
| dc.contributor.author | Fernandez, Eduardo | - |
| dc.contributor.author | Almeida, Ricardo Titze de | - |
| dc.date.accessioned | 2020-11-24T10:53:38Z | - |
| dc.date.available | 2020-11-24T10:53:38Z | - |
| dc.date.issued | 2018-07-23 | - |
| dc.identifier.citation | ALMEIDA, Simoneide Souza Titze de et al. Delivery of miRNA-targeted oligonucleotides in the
rat striatum by magnetofection with Neuromag®. Molecules, v. 23, n. 7, 1825, 2018. DOI: https://doi.org/10.3390/molecules23071825. Disponível em: https://www.mdpi.com/1420-3049/23/7/1825. Acesso em: 24 nov. 2020. | pt_BR |
| dc.identifier.uri | https://repositorio.unb.br/handle/10482/39653 | - |
| dc.language.iso | Inglês | pt_BR |
| dc.publisher | MDPI | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.title | Delivery of miRNA-targeted oligonucleotides in the rat striatum by magnetofection with Neuromag® | pt_BR |
| dc.type | Artigo | pt_BR |
| dc.subject.keyword | MicroRNA | pt_BR |
| dc.subject.keyword | Nanopartículas | pt_BR |
| dc.subject.keyword | Neuromag® | pt_BR |
| dc.rights.license | © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | pt_BR |
| dc.identifier.doi | https://doi.org/10.3390/molecules23071825 | pt_BR |
| dc.description.abstract1 | MicroRNAs (miRNAs) regulate gene expression at posttranscriptional level by triggering
RNA interference. In such a sense, aberrant expressions of miRNAs play critical roles in the
pathogenesis of many disorders, including Parkinson’s disease (PD). Controlling the level of specific
miRNAs in the brain is thus a promising therapeutic strategy for neuroprotection. A fundamental
need for miRNA regulation (either replacing or inhibition) is a carrier capable of delivering
oligonucleotides into brain cells. This study aimed to examine a polymeric magnetic particle,
Neuromag®, for delivery of synthetic miRNA inhibitors in the rat central nervous system. We injected
the miRNA inhibitor complexed with Neuromag® into the lateral ventricles next to the striatum,
by stereotaxic surgery. Neuromag efficiently delivered oligonucleotides in the striatum and
septum areas, as shown by microscopy imaging of fluorescein isothiocyanate (FITC)-labeled oligos
in astrocytes and neurons. Transfected oligos showed efficacy concerning miRNA inhibition.
Neuromag®-structured miR-134 antimiR (0.36 nmol) caused a significant 0.35 fold decrease of striatal
miR-134, as revealed by real-time quantitative polymerase chain reaction (RT-qPCR). In conclusion,
the polymeric magnetic particle Neuromag® efficiently delivered functional miRNA inhibitors in
brain regions surrounding lateral ventricles, particularly the striatum. This delivery system holds
potential as a promising miRNA-based disease-modifying drug and merits further pre-clinical studies
using animal models of PD. | pt_BR |
| dc.identifier.orcid | https://orcid.org/0000-0001-7286-1676 | pt_BR |
| dc.description.unidade | Faculdade de Agronomia e Medicina Veterinária (FAV) | - |
| Aparece nas coleções: | Artigos publicados em periódicos e afins
|
