LC–MS bioanalytical method for simultaneous determination of latanoprost and minoxidil in the skin

Oliveira, Paula M.; Sampaio, Thiago R.; França, Letícia C. F.; Gratieri, Taís; Cunha-Filho, Marcílio; Gelfuso, Guilherme Martins

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Campo DC	Valor	Lengua/Idioma
dc.contributor.author	Oliveira, Paula M.	-
dc.contributor.author	Sampaio, Thiago R.	-
dc.contributor.author	França, Letícia C. F.	-
dc.contributor.author	Gratieri, Taís	-
dc.contributor.author	Cunha-Filho, Marcílio	-
dc.contributor.author	Gelfuso, Guilherme Martins	-
dc.date.accessioned	2020-06-25T18:58:04Z	-
dc.date.available	2020-06-25T18:58:04Z	-
dc.date.issued	2020	-
dc.identifier.citation	OLIVEIRA, Paula M. et al. LC–MS bioanalytical method for simultaneous determination of latanoprost and minoxidil in the skin. Journal of Pharmaceutical and Biomedical Analysis v. 187, 113373, 2020. DOI: https://doi.org/10.1016/j.jpba.2020.113373. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S0731708520312590?via%3Dihub.	pt_BR
dc.identifier.uri	https://repositorio.unb.br/handle/10482/38195	-
dc.language.iso	Inglês	pt_BR
dc.publisher	Elsevier	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.title	LC–MS bioanalytical method for simultaneous determination of latanoprost and minoxidil in the skin	pt_BR
dc.type	Artigo	pt_BR
dc.subject.keyword	Cromatografia a líquido de alta eficiência	pt_BR
dc.subject.keyword	Espectroscopia	pt_BR
dc.subject.keyword	Medicamentos	pt_BR
dc.subject.keyword	Pele	pt_BR
dc.identifier.doi	https://doi.org/10.1016/j.jpba.2020.113373	pt_BR
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/abs/pii/S0731708520312590?via%3Dihub	pt_BR
dc.description.abstract1	The associationofminoxidil sulphate and latanoprostis currently emerging as a promising strategy for the treatment of androgenic alopecia, which is the most common type of scalp hair loss. In order to support the development of new pharmaceutical products containing such drugs combination, this study proposes a simple and efficient LC–MS bioanalytical method to simultaneously quantify minoxidil sulphate and latanoprostin different skin layers. Compounds separation was performed by liquid chromatography using a C18 column with gradient elution of a mobile phase composed of 0.1 % formic acid in acetonitrile and water at a flow rate of 0.5 mL min−1. Determinations were executed using mass spectrometry equipped with an ESI interface operating in a positive ionization mode. Quantification was performed using selective ion mode monitoring of m/z 210.1 for minoxidil sulphate and 433.3 for latanoprost. The matrix effect was very pronounced in samples containing some skin layers or electrolyte solution.Accordingly, a calibration curve for each contaminant group was built, leading to correlation coefficient values higher than 0.99. Additionally, lower limits of detection and quantification were obtained, and precision (repeatability and intermediate precision) achieved results with a coefficient of variation less than 15 %. Drug recovery from skin samples was higher than 70 %, fulfilling the recommendations. Also, the bioanalytical method was successfully tested in in vitro skin penetration studies proving its effectiveness in the development of topical formulations containing both drugs.	pt_BR
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