Título:	Anatomical and histochemical studies of the somatic embryogenesis of Syagrus oleracea from immature inflorescences
Autor(es):	Cardoso, Inaê Mariê de Araújo Silva Meira, Filipe Sathler Gomes, Ana Cristina Meneses Mendes Scherwinski-Pereira, Jonny Everson
ORCID:	http://orcid.org/0000-0001-6271-332X
Assunto:	Palmeira Embriogênese somática Morfoanatomia Histoquímica
Data de publicação:	2019
Editora:	Crop Breeding and Applied Biotechnology
Referência:	SILVA-CARDOSO, Inaê Mariê de Araújo et al. Anatomical and histochemical studies of the somatic embryogenesis of Syagrus oleracea from immature inflorescences. Crop Breeding and Applied Biotechnology, v. 19, n. 4, p. 444-450, 2019. DOI: https://doi.org/10.1590/1984-70332019v19n4n62. Disponível em: http://scielo.br/scielo.php?script=sci_arttext&pid=S1984-70332019000400444. Acesso em: 23 jan. 2020.
Abstract:	Anatomical and histochemical studies were carried out during the somatic embryogenesis of Syagrus oleracea (Mart.) Becc. from immature inflorescences. Immature rachillas were inoculated on Murashige and Skoog medium with 4.52 μM 4-amino-3,5,6-trichloropicolinic acid (Picloram) and 2,4-diclorophenoxyacetic acid. Auxin concentrations were gradually reduced for the formation of somatic embryos, while for plantlet regeneration, embryos were inoculated on a medium without growth regulator. After 270 days, two types of embryogenic calluses were observed in explants under the effect of Picloram: nodular, with a smooth surface and those without definite shape, with an irregular surface, both presenting a meristematic zone with polysaccharide content. Callus progression to the differentiation stage allowed for the conversion of somatic embryos, some with a well-defined protoderm and procambial bundles. The histochemical analysis of the somatic embryos did not identify the presence of polysaccharide and protein reserves, which emphasizes the necessity for optimization of the maturation conditions.
Licença:	(CC BY) - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI:	https://doi.org/10.1590/1984-70332019v19n4n62
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