Título:	Roles of non-coding RNA in sugarcane-microbe interaction
Autor(es):	Thiebaut, Flávia Rojas, Cristian A. Grativol, Clícia Calixto, Edmundo P. da R. Motta, Mariana R. Ballesteros, Helkin G. F. Peixoto, Barbara Lima, Berenice N. S. de Vieira, Lucas M. Walter, Maria Emília Machado Telles Armas, Elvismary M. de Entenza, Júlio O. P. Lifschitz, Sergio Farinelli, Laurent Hemerly, Adriana Silva Ferreira, Paulo Cavalcanti Gomes
Assunto:	Ácido ribonucléico Bactérias Cana-de-açúcar Biologia computacional
Data de publicação:	20-Dez-2017
Editora:	MDPI
Referência:	THIEBAUT, Flávia et al. Roles of non-coding RNA in sugarcane-microbe interaction. Non-coding RNA, v. 3, n. 4, 2013. Disponível em: <http://www.mdpi.com/2311-553X/3/4/25>. Acesso em: 26 jun. 2018. doi: https://doi.org/10.3390/ncrna3040025.
Abstract:	Studies have highlighted the importance of non-coding RNA regulation in plant-microbe interaction. However, the roles of sugarcane microRNAs (miRNAs) in the regulation of disease responses have not been investigated. Firstly, we screened the sRNA transcriptome of sugarcane infected with Acidovorax avenae. Conserved and novel miRNAs were identified. Additionally, small interfering RNAs (siRNAs) were aligned to differentially expressed sequences from the sugarcane transcriptome. Interestingly, many siRNAs aligned to a transcript encoding a coppertransporter gene whose expression was induced in the presence of A. avenae, while the siRNAs were repressed in the presence of A. avenae. Moreover, a long intergenic non-coding RNA was identified as a potential target or decoy of miR408. To extend the bioinformatics analysis, we carried out independent inoculations and the expression patterns of six miRNAs were validated by quantitative reverse transcription-PCR (qRT-PCR). Among these miRNAs, miR408—a copper- microRNA—was downregulated. The cleavage of a putative miR408 target, a laccase, was confirmed by a modified 50RACE (rapid amplification of cDNA ends) assay. MiR408 was also downregulated in samples infected with other pathogens, but it was upregulated in the presence of a beneficial diazotrophic bacteria. Our results suggest that regulation by miR408 is important in sugarcane sensing whether microorganisms are either pathogenic or beneficial, triggering specific miRNA-mediated regulatory mechanisms accordingly.
Licença:	© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
DOI:	https://doi.org/10.3390/ncrna3040025
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