Título:	Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
Autor(es):	Arruda, Mauro Maciel de Figueiredo, Fabiano Borges Marcelino, Andreza Pain Barbosa, José Ronaldo Werneck, Guilherme Loureiro Noronha, Elza Ferreira Romero, Gustavo Adolfo Sierra
Assunto:	Cão Protozoário Leishmaniose visceral Sorologia
Data de publicação:	Mar-2016
Editora:	Instituto Oswaldo Cruz, Ministério da Saúde
Referência:	ARRUDA, Mauro Maciel de et al. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 3, p. 168-173, mar. 2016. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300168&lng=en&nrm=iso>. Acesso em: 14 mar. 2018. Epub Feb 19, 2016. doi: http://dx.doi.org/10.1590/0074-02760150364.
Abstract:	In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and confirmatory methods, respectively, for the detection of canine infection. The purpose of this study was to estimate the accuracy of ELISA and IFA in parallel or serial combinations. The reference standard comprised the results of direct visualisation of parasites in histological sections, immunohistochemical test, or isolation of the parasite in culture. Samples from 98 cases and 1,327 noncases were included. Individually, both tests presented sensitivity of 91.8% and 90.8%, and specificity of 83.4 and 53.4%, for the ELISA and IFA, respectively. When tests were used in parallel combination, sensitivity attained 99.2%, while specificity dropped to 44.8%. When used in serial combination (ELISA followed by IFA), decreased sensitivity (83.3%) and increased specificity (92.5%) were observed. Serial testing approach improved specificity with moderate loss in sensitivity. This strategy could partially fulfill the needs of public health and dog owners for a more accurate diagnosis of CVL.
Unidade Acadêmica:	Faculdade de Medicina (FMD)
Licença:	Memórias do Instituto Oswaldo Cruz - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000300168&lng=en&nrm=iso. Acesso em: 14 mar. 2018.
DOI:	http://dx.doi.org/10.1590/0074-02760150364
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