Título:	Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells
Autor(es):	Mestieri, Leticia Boldrin Cornélio, Ana Lívia Gomes Rodrigues, Elisandra Márcia Salles, Loise Pedrosa Martelo, Roberta Bosso Tanomaru, Juliane Maria Guerreiro Tanomaru Filho, Mário
Assunto:	Polpa dentária Biocompatibilidade Viabilidade celular
Data de publicação:	Set-2015
Editora:	Faculdade De Odontologia De Bauru - USP
Referência:	MESTIERI, Leticia Boldrin et al. Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells. Journal of Applied Oral Science, Bauru, v. 23, n. 5, p. 467-471, set./out. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500467&lng=en&nrm=iso>. Acesso em: 29 mar. 2018. doi: http://dx.doi.org/10.1590/1678-775720150170.
Abstract:	Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus. Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs). Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%). Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure. Conclusions The hDPCs were suitable for the evaluation of new endodontic materials in vitro. MTAP may be considered a promising material for endodontic treatments.
Licença:	Journal of Applied Oral Science - This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-77572015000500467&lng=en&nrm=iso. Acesso em: 29 mar. 2018.
DOI:	http://dx.doi.org/10.1590/1678-775720150170
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