Título:	Diversity analysis of Bemisia tabaci biotypes : RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
Autor(es):	Rabello, Aline Rodrigues Queiroz, Paulo Roberto Simões, Kenya Carla Cardoso Hiragi, Cássia de Oliveira Lima, Luzia Helena C. Oliveira, Maria Regina V. Mehta, Angela
Assunto:	Mosca-branca Diversidade genética Análise filogenética
Data de publicação:	2008
Editora:	Sociedade Brasileira de Genética
Referência:	RABELLO, Aline R. et al. Diversity analysis of Bemisia tabaci biotypes: RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. Genetics and Molecular Biology, v. 31, n. 2, p. 585-590, 2008. DOI: https://doi.org/10.1590/S1415-47572008000300029. Disponível em: https://www.scielo.br/j/gmb/a/yGKfxdZC3qmRMMBCyZWsChP/abstract/?lang=en#. Acesso em: 20 maio 2022.
Abstract:	The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci.
Unidade Acadêmica:	Faculdade de Agronomia e Medicina Veterinária (FAV)
Licença:	This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI:	https://doi.org/10.1590/S1415-47572008000300029
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