Título:	Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4
Autor(es):	Miller, Robert Neil Gerard Passos, Marco Aurélio Ninômia Menezes, Natalia N. P. Souza Júnior, Manoel T. Costa, Marcos M. do Carmo Azevedo, Vânia Cristina Rennó Amorim, Edson Perito Pappas Júnior, Georgios Joannis Ciampi, Ana Y.
Assunto:	Banana - cultivo Banana - genética
Data de publicação:	27-Mai-2010
Editora:	BioMed Central
Referência:	MILLER, Robert Neil Gerard et al. Characterization of novel microsatellite markers in Musa acuminata subsp. burmannicoides, var. Calcutta 4. BMC Research Notes, v. 3, Article 148, 27 mai. 2010. Disponível em: <https://bmcresnotes.biomedcentral.com/articles/10.1186/1756-0500-3-148>. Acesso em: 24 jul. 2017. doi: https://doi.org/10.1186/1756-0500-3-148.
Abstract:	Background Banana is a nutritionally important crop across tropical and sub-tropical countries in sub-Saharan Africa, Central and South America and Asia. Although cultivars have evolved from diploid, triploid and tetraploid wild Asian species of Musa acuminata (A genome) and Musa balbisiana (B genome), many of today's commercial cultivars are sterile triploids or diploids, with fruit developing via parthenocarpy. As a result of restricted genetic variation, improvement has been limited, resulting in a crop frequently lacking resistance to pests and disease. Considering the importance of molecular tools to facilitate development of disease resistant genotypes, the objectives of this study were to develop polymorphic microsatellite markers from BAC clone sequences for M. acuminata subsp. burmannicoides, var. Calcutta 4. This wild diploid species is used as a donor cultivar in breeding programs as a source of resistance to diverse biotic stresses. Findings Microsatellite sequences were identified from five Calcutta 4 BAC consensi datasets. Specific primers were designed for 41 loci. Isolated di-nucleotide repeat motifs were the most abundant, followed by tri-nucleotides. From 33 tested loci, 20 displayed polymorphism when screened across 21 diploid M. acuminata accessions, contrasting in resistance to Sigatoka diseases. The number of alleles per SSR locus ranged from two to four, with a total of 56. Six repeat classes were identified, with di-nucleotides the most abundant. Expected heterozygosity values for polymorphic markers ranged from 0.31 to 0.75. Conclusions This is the first report identifying polymorphic microsatellite markers from M. acuminata subsp. burmannicoides, var. Calcutta 4 across accessions contrasting in resistance to Sigatoka diseases. These BAC-derived polymorphic microsatellite markers are a useful resource for banana, applicable for genetic map development, germplasm characterization, evolutionary studies and marker assisted selection for traits.
Licença:	© Miller et al; licensee BioMed Central Ltd. 2010 This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI:	https://dx.doi.org/10.1186/1756-0500-3-148
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