Título:	A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease
Autor(es):	Santiago, Paula Beatriz Assumpção, Teresa Cristina França de Araújo, Carla Nunes de Bastos, Izabela Marques Dourado Neves, David Silva, Ionizete Garcia da Charneau, Sébastien Queiroz, Rayner Myr Lauterjung Raiol, Tainá Oliveira, João Victor de Araújo Sousa, Marcelo Valle de Calvo, Eric Ribeiro, José M. C. Santana, Jaime Martins de
Assunto:	Triatoma Chagas, Doença de Saliva Proteínas
Data de publicação:	29-Abr-2016
Editora:	Plos One
Referência:	SANTIAGO, Paula Beatriz et al. A Deep Insight into the Sialome of Rhodnius neglectus, a vector of chagas disease. Plos Neglected Tropical Diseases, v. 10, n. 4, Article e0004581, 29 abr. 2016. Disponível em: <http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581>. Acesso em: 21 jun. 217. doi: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004581.
Abstract:	Background Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immuneresponses. Methods/Principal Findings Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva.We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. Conclusions/Significance The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.
Licença:	Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
DOI:	https://dx.doi.org/10.1371/journal.pntd.0004581
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