| Campo DC | Valor | Lengua/Idioma |
|---|
| dc.contributor.author | Nóbrega, Otávio de Tolêdo | - |
| dc.contributor.author | Santana, Jaime Martins de | - |
| dc.contributor.author | Sturm, Nancy Ritsuko | - |
| dc.contributor.author | Teixeira, Antonio Raimundo Lima Cruz | - |
| dc.contributor.author | Campbell, David A. | - |
| dc.date.accessioned | 2012-12-06T18:03:02Z | - |
| dc.date.available | 2012-12-06T18:03:02Z | - |
| dc.date.issued | 2004-08 | - |
| dc.identifier.citation | NOBREGA, Otávio T. et al. HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000500010&lng=pt&nrm=iso>. Acesso em: 4 dez. 2012. doi:
http://dx.doi.org/10.1590/S0074-02762004000500010. | en |
| dc.identifier.uri | http://repositorio.unb.br/handle/10482/11768 | - |
| dc.language.iso | Inglês | en |
| dc.publisher | Instituto Oswaldo Cruz, Ministério da Saúde | en |
| dc.rights | Acesso Aberto | en |
| dc.title | HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi | en |
| dc.type | Artigo | en |
| dc.subject.keyword | Tripanossoma cruzi | en |
| dc.subject.keyword | Vetores artrópodes | en |
| dc.subject.keyword | Vetores - controle | en |
| dc.rights.license | Instituto Oswaldo Cruz, Ministério da Saúde - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: http://www.scielo.br/scielo.php?script=sci_serial&pid=0074-0276&lng=pt&nrm=iso. Acesso em: 4 dez. 2012. | en |
| dc.identifier.doi | http://dx.doi.org/10.1590/S0074-02762004000500010 | en |
| dc.description.abstract1 | This work describes the development and functional testing of two episomes for stable transfection of Trypanosoma cruzi. pHygD contained the 5'- and 3'- flanking regions of the gene encoding the cathepsin B-like protease of T. cruzi as functional trans-splicing and polyadenylation signals for the hygR ORF. Evidence is presented to support extrachromosomal maintenance and organization as tandem repeats in transfected parasites. pPac was derived from pHygD by replacement of the entire hygR ORF with a purR coding region. The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi. | - |
| dc.description.unidade | Faculdade UnB Ceilândia (FCE) | - |
| dc.description.unidade | Curso de Saúde Coletiva (FCE-SC) | - |
| Aparece en las colecciones: | Artigos publicados em periódicos e afins
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