Skip navigation
Please use this identifier to cite or link to this item: http://repositorio.unb.br/handle/10482/6327
Files in This Item:
File Description SizeFormat 
ARTIGO_HistopathologicEvaluationPeritoneum.pdf566,33 kBAdobe PDFView/Open
Title: Histopathologic evaluation of the peritoneum exposed to heat shock. Experimental study in rats
Other Titles: Avaliação histopatológica do peritônio exposto a choque térmico. Estudo experimental em ratos
Authors: Lopes, João Vieira
Oliveira, Paulo Gonçalves de
Sousa, João Batista de
Báo, Sônia Nair
Takano, Gustavo Henrique Soares
Leal, Isabel Irene Rama
Assunto:: Choque térmico
Lavagem peritoneal
Peritonite
Ratos
Issue Date: 2007
Citation: LOPES, João Vieira et al. Histopathologic evaluation of the peritoneum exposed to heat shock: experimental study in rats. Acta cirúrgica brasileira, v.22, n.5, p.342-350, 2007. Disponível em: <http://www.scielo.br/pdf/acb/v22n5/04.pdf>. Acesso em: 8 nov. 2010. doi: 10.1590/S0102-86502007000500004.
Abstract: PURPOSE: To evaluate histopathologic alterations of the peritoneum exposed to heat shock. METHODS: Sixty rats were randomly distributed into 6 groups: Heat Shock (HS), High Temperature (HT), Body Temperature (BT), Temperature 0oC (TZ), Sham (SH) and Control (CG) with 10 animals each. The peritoneal cavity of animals from groups HS, HT, BT and TZ was irrigated with NaCl solution 0.9% at temperatures 50ºC, 0ºC, 50ºC, 37ºC and 0ºC, respectively. For animals from group SH, the procedures were simulated and those from group CG, laparotomy and biopsies were conducted. Twenty-four hours later, biopsies of the peritoneum for exams under light and electronic microscopy were performed. RESULTS: Edema was found in groups HS 80%, HT 60%, BT 30% TZ 70%, SH 40% and CG 30%. Vascular congestion was found in groups HS 20%, HT 30%, BT 10% and TZ 20%. Erythrocyte extravasation was found in groups HT 60% and SH 10%. Mesothelium destruction was found in 100% of specimens from groups HS, HT, BT, TZ, SH and CG 90%. Necrosis was found in groups HS 30%, HT 20% and BT 10%. The mean peritoneal thickness ranged from 42.26 µm (TZ) to 26.42 µm (CG). CONCLUSION: The heat shock caused no deaths, but promoted significant peritoneal edema without affecting the other histopathologic indicatives. _______________________________________________________________________________ RESUMO
OBJETIVO: Avaliar alterações histopatológicas do peritônio exposto a choque térmico. MÉTODOS: Sessenta ratos foram distribuídos aleatoriamente em seis grupos: Choque Térmico (CT), Temperatura Elevada (TE), Temperatura 0ºC (TZ) Sham (SH) e Controle (GC) com 10 animais. A cavidade peritoneal dos animais dos grupos CT, TE, TC e TZ foi irrigada com solução de NaCl 0,9% nas temperaturas, 50ºC e 0ºC, 50ºC, 37ºC e 0ºC, respectivamente. Nos animais do grupo SH foram simulados os procedimentos e nos do GC laparotomia e biópsias. Depois de 24 horas foram realizadas biópsias do peritônio para exames sob microscopia de luz e eletrônica. RESULTADOS: Edema foi encontrado nos grupos CT 80%, TE 60%, TC 30%, TZ 70%, SH 40% e GC 30%. Congestão vascular foi encontrada nos grupos CT 20%, TE 30%, TC 10% e TZ 20%. Extravasamento de hemácias foi encontrado nos grupos TE 60% e SH 10%. Destruição de mesotélio foi encontrada em 100% dos espécimes dos grupos CT, TE, TC, TZ, SH e no grupo GC 90%. Necrose foi encontrada nos grupos CT 30%, TE 20% e TC 10%. A espessura média do peritônio variou de 42,26 µm (TZ) a 26,42 µm (GC). CONCLUSÃO: O choque térmico não causou óbitos, mas promoveu edema peritoneal significante sem alterar os demais indicadores histopatológicos.
DOI: https://dx.doi.org/10.1590/S0102-86502007000500004
Appears in Collections:CEL - Artigos publicados em periódicos

Show full item record Recommend this item " class="statisticsLink btn btn-primary" href="/handle/10482/6327/statistics">



This item is licensed under a Creative Commons License Creative Commons