Skip navigation
Use este identificador para citar ou linkar para este item: http://repositorio.unb.br/handle/10482/33831
Arquivos associados a este item:
Não existem arquivos associados a este item.
Título: Sexual transmission of American Trypanosomes from males and females to naive mates
Autor(es): Almeida, Adriana B.
Araújo, Perla F.
Bernal, Francisco Ernesto Moreno
Rosa, Ana de Cassia
Valente, Sebastião A.
Teixeira, Antonio Raimundo Lima Cruz
Assunto: Tripanossoma cruzi
Chagas, Doença de
Data de publicação: 2019
Editora: JoVE
Referência: ALMEIDA, Adriana B. et al. Sexual transmission of American Trypanosomes from males and females to naive mates. J. Vis. Exp., n. 143, e57985, 2019. DOI: 10.3791/57985. Disponível em: https://www.jove.com/video/57985/sexual-transmission-american-trypanosomes-from-males-females-to-naive. Acesso em: 29 jan. 2019.
Abstract: American trypanosomiasis is transmitted to humans by triatomine bugs through the ingestion of contaminated food, by blood transfusions or accidently in hospitals and research laboratories. In addition, the Trypanosoma cruzi infection is transmitted congenitally from a chagasic mother to her offspring, but the male partner's contribution to in utero contamination is unknown. The findings of nests and clumps of amastigotes and of trypomastigotes in the theca cells of the ovary, in the goniablasts and in the lumen of seminiferous tubules suggest that T. cruzi infections are sexually transmitted. The research protocol herein presents the results of a family study population showing parasite nuclear DNA in the diploid blood mononuclear cells and in the haploid gametes of human subjects. Thus, three independent biological samples collected one year apart confirmed that T. cruzi infections were sexually transmitted to progeny. Interestingly, the specific T. cruzi antibody was absent in the majority of family progeny that bore immune tolerance to the parasite antigen. Immune tolerance was demonstrated in chicken refractory to T. cruzi after the first week of embryonic growth, and chicks hatched from the flagellate-inoculated eggs were unable to produce the specific antibody. Moreover, the instillation of the human semen ejaculates intraperitoneally or into the vagina of naive mice yielded T. cruzi amastigotes in the epididymis, seminiferous tubule, vas deferens and uterine tube with an absence of inflammatory reactions in the immune privileged organs of reproduction. The breeding of T. cruzi-infected male and female mice with naive mates resulted in acquisition of the infections, which were later transmitted to the progeny. Therefore, a robust education, information and communication program that involves the population and social organizations is deemed necessary to prevent Chagas disease.
DOI: 10.3791/57985
Link: https://www.jove.com/video/57985
Aparece nas coleções:FMD - Artigos publicados em periódicos

Mostrar registro completo do item Recomendar este item Visualizar estatísticas



Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.