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Title: Cinerascetins, new peptides from Hypsiboas cinerascens : MALDI LIFT-TOF-MS/MS de novo sequence and imaging analysis
Authors: Almeida, Richardson Alves de
Gordo, Marcelo
Silva, Felipe Moura Araujo da
Araújo, Rafael C. de
Ramada, Marcelo Henrique Soller
Abrão, Fernando Yano
Costa, Túlio O. G.
Koolen, Hector Henrique Ferreira
Souza, Afonso Duarte Leão de
Bloch Júnior, Carlos
Assunto:: Anuro
Peptídeos antimicrobianos
Secreção cutânea
Issue Date: Nov-2015
Publisher: Sociedade Brasileira de Química
Citation: ALMEIDA, Richardson Alves de et al. Cinerascetins, new peptides from Hypsiboas cinerascens: MALDI LIFT-TOF-MS/MS de novo sequence and imaging snalysis. Journal of the Brazilian Chemical Society, São Paulo, v. 26, n. 11, p. 2290-2297, nov. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001102290&lng=en&nrm=iso>. Acesso em: 16 abr. 2018. doi: http://dx.doi.org/10.5935/0103-5053.20150219.
Abstract: The continuous search for antimicrobial candidates pushes the pursuit of compounds in the most diverse organisms. Amphibians are known as a prolific source of antibacterial peptides. Based on the rich biodiversity of the Amazon region the unexplored green-tree frog (Hypsiboas cinerascens) was studied for its skin secretion peptide content. Chromatographic separations and established tandem mass spectrometry (MS/MS) methods were used for sequencing the primary structures of the purified compounds. De novo sequencing lead to the identification of five new peptides related to hylaseptin P1, displaying an aminated C-terminal. Sequencing of the complementary deoxyribonucleic acid (cDNA) analysis allowed the disambiguation of isobaric amino-acids for C-01. Matrix assisted laser desorption ionization (MALDI) was carried out, demonstrating the in situ co-occurrence of the identified peptides in the dorsal skin. The major peptide C-01 was synthesized and assayed against a selection of microorganisms displaying minimal inhibitory concentrations (MICs) ranging from 4 to 16 µM.
Licença:: Journal of the Brazilian Chemical Society - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532015001102290&lng=en&nrm=iso. Acesso em: 16 abr. 2018.
DOI: http://dx.doi.org/10.5935/0103-5053.20150219
Appears in Collections:CEL - Artigos publicados em periódicos

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