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Title: The circulation of human astrovirus genotypes in the Central West Region of Brazil
Authors: Silva, Paula Andreia
Santos, Rodrigo Alessandro Tôgo
Costa, Paulo Sérgio Sucasas
Teixeira, José Marcus Sócrates
Giugliano, Loreny Gimenes
Andreasi, Márcia Sueli Assis
Leite, José Paulo Gagliardi
Schreier, Ecakart
Cardoso, Divina das Dôres de Paula
Assunto:: astrovirus
genotyping
sequencing
nested-PCR
Brazil
Issue Date: 2009
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Mem. Inst. Oswaldo Cruz,v.104,n.4,p.655-658,2009
Abstract: Out of 1,588 faecal samples of children taken from three locations of the Central West Region of Brazil, 57 were positive for astroviruses (HAstVs) using reverse transcription-polymerase chain reaction (RT-PCR). They were genotyped by nested RT-PCR and/or genomic sequencing. HAstV-1 (42.8%), HAstV-2 (23.2%), HAstV-3 (3.6%), HAstV-4 (14.3%) and HAstVs -5, -6, -7 and -8 (1.8% each) were detected. In Goiânia and Campo Grande, HAstV-1 was the most frequently detected genotype while in Brasília (DF) it was HAstV-2. Shifts in the circulation of astrovirus genotypes were observed in DF and Campo Grande. All samples collected by rectal swabs were viral negative. The astrovirus genotypes were detected in all age groups and there was no correlation between genotype and age group.
DOI: https://dx.doi.org/10.1590/S0074-02762009000400021
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