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Title: Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil
Authors: Borges, Ana Maria Tavares
Teixeira, José Marcus Sócrates
Costa, Paulo Sérgio Sucasas da
Giugliano, Loreny Gimenes
Fiaccadori, Fabíola Souza
Franco, Rachel de Carvalho e
Brito, Wilia Marta Elsner Diederichsen de
Leite, José Paulo Gagliardi
Cardoso, Divina das Dores de Paula
Assunto:: calicivirus
acute gastroenteritis
reverse transcriptase
polymerase chain reaction
Issue Date: 2006
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: Mem. Inst. Oswaldo Cruz,v.101,n.7,p.721-724,2006
Abstract: The objective of this study was to describe the circulation of caliciviruses in the West Central region of Brazil and its correlation with children's gender and age, as well as with the year and months of the sample collection. Reverse transcriptase-polymerase chain reaction was performed to detect the human calicivirus genome in 1006 fecal samples that were collected in Goiânia (n = 696) and Brasília (n = 310). Viral RNA was detected in 8.6% of the samples. No significant difference in viral prevalence was found regarding gender, age or year of the sample. However, it was observed that in Goiânia, there is a higher incidence of caliciviruses from September to March. The analysis employing three primer pairs demonstrated that the Ni/E3 or JV12/13 primer pairs, which detect norovirus (NoV), detected 41 positive samples while the 289/290 primer pair, which detects NoV or sapovirus, detected the remaining 46 samples. Calicivirus circulates in the West Central region of Brazil and for better detection of this virus it is important to use more than one primer pair. Also, we conclude that the seasonality presented by this virus is related to higher humidity in the period.
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