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Title: MicroRNA expression profiles in human CD3+ T cells following stimulation with anti‑human CD3 antibodies
Authors: Sousa, Isabel Garcia
Almo, Manuela Maragno do
Simi, Kelly Cristina Rodrigues
Bezerra, Maryani Andressa Gomes
Andrade, Rosângela Vieira
Maranhão, Andréa Queiroz
Brígido, Marcelo de Macedo
Assunto:: Anticorpos
Imunologia
Issue Date: 14-Mar-2017
Publisher: BioMed Central
Citation: SOUSA, Isabel Garcia et al. MicroRNA expression profiles in human CD3+ T cells following stimulation with anti‑human CD3 antibodies. BMC Res Notes, v. 10, Article 124, 14 mar. 2017. Disponível em: <https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2442-y>. Acesso em: 27 jun. 2017. doi: https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-017-2442-y.
Abstract: Background: Anti-CD3 therapy can induce immunosuppression by several non mutually exclusive mechanisms that have been proposed to explain the therapeutic effect the administration anti-CD3 mAb, but its immunoregulatory mechanism is still not completely clear. In T cells, microRNAs (miRNAs) regulate several pathways, including those associated with immune tolerance. Here, we report changes in miRNA expression in T cells following treatment with anti-human CD3 antibodies. Peripheral blood mononuclear cells were cultured in the presence of the monoclonal antibody OKT3 or a recombinant fragment of humanized anti-CD3. Following these treatments, the expression profiles of 31 miRNA species were assessed in T cells using TaqMan arrays. Results: Eight of the tested miRNAs (miR-155, miR-21, miR-146a, miR-210, miR-17, miR-590-5p, miR-106b and miR- 301a) were statistically significantly up- or down-regulated relative to untreated cells. Conclusions: Stimulation of T cells with anti-human CD3 antibodies alters miRNA expression patterns, including of miRNA species associated with immune regulatory pathways.
Licença:: © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
DOI: 10.1186/s13104-017-2442-y
Appears in Collections:CEL - Artigos publicados em periódicos

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