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Title: Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene
Authors: Guimarães, Larissa Arrais
Mota, Ana Paula Zotta
Araújo, Ana Cláudia Guerra
Figueiredo, Lúcio Flávio de Alencar
Pereira, Bruna Medeiros
Saraiva, Mario Alfredo de Passos
Silva, Raquel Bispo
Danchin, Etienne G. J.
Guimarães, Patrícia Messenberg
Brasileiro, Ana Cristina Miranda
Assunto:: Proteínas
Radiação ultravioleta
Soja
Issue Date: 27-Feb-2017
Publisher: US National Library of Medicine Institutos Nacionais de Saúde
Citation: GUIMARAES, Larissa Arrais et al. Genome-wide analysis of expansin superfamily in wild Arachis discloses a stress-responsive expansin-like B gene. Plant Molecular Biology, v. 94, p. 79-96, 27 fev. 2017. Disponível em: <https://www.ncbi.nlm.nih.gov/pubmed/28243841>. Acesso em: 6 jul. 2017. doi: https://www.ncbi.nlm.nih.gov/pubmed/28243841.
Abstract: Expansins are plant cell wall-loosening proteins involved in adaptive responses to environmental stimuli and various developmental processes. The first genome-wide analysis of the expansin superfamily in the Arachis genus identified 40 members in A. duranensis and 44 in A. ipaënsis, the wild progenitors of cultivated peanut (A. hypogaea). These expansins were further characterized regarding their subfamily classification, distribution along the genomes, duplication events, molecular structure, and phylogeny. A RNA-seq expression analysis in different Arachis species showed that the majority of these expansins are modulated in response to diverse stresses such as water deficit, rootknot nematode (RKN) infection, and UV exposure, with an expansin-like B gene (AraEXLB8) displaying a highly distinct stress-responsive expression profile. Further analysis of the AraEXLB8 coding sequences showed high conservation across the Arachis genotypes, with eight haplotypes identified. The modulation of AraEXLB8 expression in response to the aforementioned stresses was confirmed by qRT-PCR analysis in distinct Arachis genotypes, whilst in situ hybridization revealed transcripts in different root tissues according to the stress imposed. The overexpression of AraEXLB8 in soybean (Glycine max) composite plants remarkably decreased the number of galls in transformed hairy roots inoculated with RKN. This study improves the current understanding of the molecular evolution, divergence, and gene expression of expansins in Arachis, and provides molecular and functional insights into the role of expansin-like B, the less-studied plant expansin subfamily.
Licença:: © The Author(s) 2017. This article is published with open access at Springerlink.com - Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
DOI: https://dx.doi.org/10.1007/s11103-017-0594-8
Appears in Collections:BOT - Artigos publicados em periódicos

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