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Title: Antifungal activity of amphotericin B conjugated to nanosized magnetite in the treatment of paracoccidioidomycosis
Authors: Saldanha, Camila Arruda
Garcia, Mônica Pereira
Iocca, Diego Cesar
Rebelo, Luciana Guilherme
Souza, Ana Camila Oliveira
Bocca, Anamélia Lorenzetti
Santos, Maria de Fátima Menezes Almeida
Morais, Paulo Cesar
Azevedo, Ricardo Bentes de
Assunto:: Fungicidas
Testes
Fármacos
Issue Date: 15-Jun-2016
Publisher: Plos One
Citation: SALDANHA, Camila Arruda. et al. Antifungal activity of amphotericin B conjugated to nanosized magnetite in the treatment of paracoccidioidomycosis. Plos One Neglected Tropical Diseases, v. 10, n. 6, Article e0004754, 15 jun. 2016. Disponível em: <http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004754>. Acesso em: 9 jun. 2017. doi: http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004754.
Abstract: This study reports on in vitro and in vivo tests that sought to assess the antifungal activity of a newly developed magnetic carrier system comprising amphotericin B loaded onto the surface of pre-coated (with a double-layer of lauric acid) magnetite nanoparticles. The in vitro tests compared two drugs; i.e., this newly developed form and free amphotericin B. We found that this nanocomplex exhibited antifungal activity without cytotoxicity to human urinary cells and with low cytotoxicity to peritoneal macrophages. We also evaluated the efficacy of the nanocomplex in experimental paracoccidioidomycosis. BALB/c mice were intratracheally infected with Paracoccidioides brasiliensis and treated with the compound for 30 or 60 days beginning the day after infection. The newly developed amphotericin B coupled with magnetic nanoparticles was effective against experimental paracoccidioidomycosis, and it did not induce clinical, biochemical or histopathological alterations. The nanocomplex also did not induce genotoxic effects in bone marrow cells. Therefore, it is reasonable to believe that amphotericin B coupled to magnetic nanoparticles and stabilized with bilayer lauric acid is a promising nanotool for the treatment of the experimental paracoccidioidomycosis because it exhibited antifungal activity that was similar to that of free amphotericin B, did not induce adverse effects in therapeutic doses and allowed for a reduction in the number of applications.
Licença:: Copyright: © 2016 Saldanha et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: https://dx.doi.org/10.1371/journal.pntd.0004754
Appears in Collections:CEL - Artigos publicados em periódicos

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