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Title: Free rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy
Authors: Carneiro, Marcella Lemos Brettas
Nunes, Eloiza da Silva
Peixoto, Raphael Cândido Apolinário
Oliveira, Ricardo G. Simões
Lourenço, Luiza Helena Madia
Silva, Izabel C.R. da
Simioni, Andreza Ribeiro
Tedesco, Antonio Claudio
Souza, Aparecido R de
Lacava, Zulmira Guerrero Marques
Báo, Sônia Nair
Assunto:: Mamas - câncer - tratamento
Quimioterapia
Issue Date: 2011
Publisher: Jornal de Nanobiotecnologia
Citation: CARNEIRO, Marcella Lemos Brettas, et al. Free rhodium (II) citrate and rhodium (II) citrate magnetic carriers as potential strategies for breast cancer therapy. Journal of Nanobiotechnology, v. 9, p. 11, 2011. Disponível em:<http://www.jnanobiotechnology.com/content/9/1/11>. Acesso em: 03 fev. 2015.
Abstract: Background: Rhodium (II) citrate (Rh2(H2cit)4) has significant antitumor, cytotoxic, and cytostatic activity on Ehrlich ascite tumor. Although toxic to normal cells, its lower toxicity when compared to carboxylate analogues of rhodium (II) indicates Rh2(H2cit)4 as a promising agent for chemotherapy. Nevertheless, few studies have been performed to explore this potential. Superparamagnetic particles of iron oxide (SPIOs) represent an attractive platform as carriers in drug delivery systems (DDS) because they can present greater specificity to tumor cells than normal cells. Thus, the association between Rh2(H2cit)4 and SPIOs can represent a strategy to enhance the former’s therapeutic action. In this work, we report the cytotoxicity of free rhodium (II) citrate (Rh2(H2cit)4) and rhodium (II) citrate-loaded maghemite nanoparticles or magnetoliposomes, used as drug delivery systems, on both normal and carcinoma breast cell cultures. Results: Treatment with free Rh2(H2cit)4 induced cytotoxicity that was dependent on dose, time, and cell line. The IC50 values showed that this effect was more intense on breast normal cells (MCF-10A) than on breast carcinoma cells (MCF-7 and 4T1). However, the treatment with 50 μM Rh2(H2cit)4-loaded maghemite nanoparticles (Magh-Rh2(H2cit)4) and Rh2(H2cit)4-loaded magnetoliposomes (Lip-Magh-Rh2(H2cit)4) induced a higher cytotoxicity on MCF-7 and 4T1 than on MCF-10A (p < 0.05). These treatments enhanced cytotoxicity up to 4.6 times. These cytotoxic effects, induced by free Rh2(H2cit)4, were evidenced by morphological alterations such as nuclear fragmentation, membrane blebbing and phosphatidylserine exposure, reduction of actin filaments, mitochondrial condensation and an increase in number of vacuoles, suggesting that Rh2(H2cit)4 induces cell death by apoptosis. Conclusions: The treatment with rhodium (II) citrate-loaded maghemite nanoparticles and magnetoliposomes induced more specific cytotoxicity on breast carcinoma cells than on breast normal cells, which is the opposite of the results observed with free Rh2(H2cit)4 treatment. Thus, magnetic nanoparticles represent an attractive platform as carriers in Rh2(H2cit)4 delivery systems, since they can act preferentially in tumor cells. Therefore, these nanopaticulate systems may be explored as a potential tool for chemotherapy drug development.
Licença:: Este é um artigo Open Access distribuído sob os termos da Licença Creative Commons Attribution (http://creativecommons.org/licenses/by/2.0 ), que permite o uso irrestrito, distribuição e reprodução em qualquer meio, desde que a obra original é devidamente citada. Fonte:<http://www.jnanobiotechnology.com/content/9/1/11>. Acesso em: 03 fev. 2015.
Appears in Collections:CEL - Artigos publicados em periódicos

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