Skip navigation
Use este identificador para citar ou linkar para este item: http://repositorio.unb.br/handle/10482/12640
Arquivos associados a este item:
Arquivo Descrição TamanhoFormato 
ARTIGO_ComparativeGenomicAnalysis.pdf966,59 kBAdobe PDFVisualizar/Abrir
Título: Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis
Autor(es): Desjardins, Christopher A.
Champion, Mia D.
Holder, Jason W.
Muszewska, Anna
Goldberg, Jonathan
Bailão, Alexandre Melo
Brígido, Marcelo de Macedo
Ferreira, Márcia Eliana da Silva
Garcia, Ana Maria
Grynberg, Marcin
Gujja, Sharvari
Heiman, David I.
Henn, Matthew R.
Kodira, Chinnappa D.
León-Narváez, Henry
Longo, Larissa Valle Guilhen
Ma, Li-Jun
Malavazi, Iran
Matsuo, Alisson Leonardo
Morais, Flávia Villaça
Pereira, Maristela
Rodríguez-Brito, Sabrina
Sakthikumar, Sharadha
Salem-Izacc, Sílvia Maria
Sykes, Sean M.
Teixeira, Marcus de Melo
Vallejo, Milene Carmes
Walter, Maria Emília Machado Telles
Yandava, Chandri
Young, Sarah
Zeng, Qiandong
Zucker, Jeremy
Felipe, Maria Sueli Soares
Goldman, Gustavo Henrique
Haas, Brian J.
McEwen, Juan G.
Nino-Vega, Gustavo
Puccia, Rosana
San-Blas, Gioconda
Soares, Célia Maria de Almeida
Birren, Bruce W.
Cuomo, Christina A.
Assunto: Paracoccidioides brasiliensis
Fungos patogênicos
Paracoccidioidomicose
Genética molecular
Data de publicação: Out-2011
Editora: PLOS
Referência: DESJARDINS, Christopher A. et al. Comparative genomic analysis of human fungal pathogens causing Paracoccidioidomycosis. PLOS Genetics, v. 7, n. 10, out. 2011. Disponível em: <http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1002345>. Acesso em: 21 mar. 2013. DOI: 10.1371/journal.pgen.1002345.
Resumo: Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. In addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.
Licença: © 2011 Desjardins et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: http://www.plosgenetics.org/article/info%3Adoi%2F10.1371%2Fjournal.pgen.1002345. Acesso em: 21 mar. 2013.
DOI: 10.1371/journal.pgen.1002345
Aparece nas coleções:CEL - Artigos publicados em periódicos

Mostrar registro completo do item Recomendar este item Visualizar estatísticas



Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.