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Title: HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi
Authors: Nóbrega, Otávio de Tolêdo
Santana, Jaime Martins de
Sturm, Nancy Ritsuko
Teixeira, Antonio Raimundo Lima Cruz
Campbell, David A.
Assunto:: Tripanossoma cruzi
Vetores artrópodes
Vetores - controle
Issue Date: Aug-2004
Publisher: Instituto Oswaldo Cruz, Ministério da Saúde
Citation: NOBREGA, Otávio T. et al. HygR and PurR plasmid vectors for episomal transfection of Trypanosoma cruzi. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 5, ago. 2004. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000500010&lng=pt&nrm=iso>. Acesso em: 4 dez. 2012. doi: http://dx.doi.org/10.1590/S0074-02762004000500010.
Abstract: This work describes the development and functional testing of two episomes for stable transfection of Trypanosoma cruzi. pHygD contained the 5'- and 3'- flanking regions of the gene encoding the cathepsin B-like protease of T. cruzi as functional trans-splicing and polyadenylation signals for the hygR ORF. Evidence is presented to support extrachromosomal maintenance and organization as tandem repeats in transfected parasites. pPac was derived from pHygD by replacement of the entire hygR ORF with a purR coding region. The ability to modify pHygD and the availability of the complete DNA sequence make these plasmids useful tools for the genetic manipulation of T. cruzi.
Licença:: Instituto Oswaldo Cruz, Ministério da Saúde - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte: http://www.scielo.br/scielo.php?script=sci_serial&pid=0074-0276&lng=pt&nrm=iso. Acesso em: 4 dez. 2012.
DOI: http://dx.doi.org/10.1590/S0074-02762004000500010
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