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Title: Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis
Authors: Lunardi, Claure Nain
Rotta, Jeane Cristina Gomes
Tedesco, Antonio Claudio
Assunto:: Óxido nítrico
Terapia fotodinâmica
Raios laser
Issue Date: May-2003
Publisher: Associação Brasileira de Divulgação Científica
Citation: ROTTA, Jeane Cristina Gomes; LUNARDI, Claure Nain; TEDESCO, Antonio Claudio. Nitric oxide release from the S-nitrosothiol zinc phthalocyanine complex by flash photolysis. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 36, n. 5, maio 2003. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000500005&lng=pt&nrm=iso>. Acesso em: 08 nov. 2012. http://dx.doi.org/10.1590/S0100-879X2003000500005.
Abstract: The photogeneration of nitric oxide (NO) using laser flash photolysis was investigated for S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NacySNO) at pH 6.4 (PBS/HCl) and 7.4 (PBS). Irradiation of S-nitrosothiol with light ( = 355 nm followed by absorption spectroscopy) resulted in the homolytic decomposition of NacySNO and GSNO to generate radicals (GS• and NacyS•) and NO. The release of NO from donor compounds measured with an ISO-Nometer apparatus was larger at pH 7.4 than pH 6.4. NacySNO was also incorporated into dipalmitoyl-phosphatidylcholine liposomes in the presence and absence of zinc phthalocyanine (ZnPC), a well-known photosensitizer useful for photodynamic therapy. Liposomes are usually used as carriers for hydrophobic compounds such as ZnPC. Inclusion of ZnPC resulted in a decrease in NO liberation in liposomal medium. However, there was a synergistic action of both photosensitizers and S-nitrosothiols resulting in the formation of other reactive species such as peroxynitrite, which is a potent oxidizing agent. These data show that NO release depends on pH and the medium, as well as on the laser energy applied to the system. Changes in the absorption spectrum were monitored as a function of light exposure.
Licença:: Brazilian Journal of Medical and Biological Research - Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons (Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)). Fonte:http://www.scielo.br/scielo.php?script=sci_serial&pid=0100-879X&lng=pt&nrm=iso. Acesso em: 8 nov. 2012.
DOI: http://dx.doi.org/10.1590/S0100-879X2003000500005
Appears in Collections:FCE-FAR - Artigos publicados em periódicos

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